Wybierz:

Polimeraza DNA phi29 (EN20)

0.00

Out of stock

Polimeraza DNA phi29 (EN20)

Wyczyść

Phi29 Polymerase is highly processive recombinant polymerase with exceptional strand displacement activity, which allows for highly efficient isothermal DNA amplification. The enzyme is capable of up to 70 thousands base insertions per binding event. The polymerase also possesses a 3’→ 5’ proofreading exonuclease activity acting preferentially on ssDNA or RNA. Extremely high yields of amplified DNA can be obtained even from minute amounts of template. EN20, EN20-10, EN20-010, EN20-50, EN20-050

Opis

phi29 DNA Polymerase is a highly processive recombinant polymerase with exceptional strand displacement activity, which allows for highly efficient isothermal DNA amplification. The enzyme is capable of up to 70 thousands base insertions per binding event. The polymerase also possesses a 3’to 5’ proofreading exonuclease activity acting preferentially on ssDNA or RNA. Therefore 3′-modified primers are recommended. Features Recombinant polymerase derived from the Bacillus subtilis phage phi29 and over-expressed in E. coli. Extremely processive polymerase, enabling amplification up to 70 kbp. Extremely high yields of amplified DNA can be obtained even from minute amounts of template. High-Fidelity polymerase – possesses a 3’-5’ exonuclease (proofreading) activity acting preferentially on ssDNA or RNA. Isothermal polymerase – no need for thermal cycling. Due to heat lability of the enzyme, it is possible to use the amplification products directly in the downstream applications  

Protokół & Ulotka

Załącznik 1

Jakość i bezpieczeństwo

Załącznik 1

Literatura

Załącznik 1

×
×

Koszyk