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AMPLIFYME SG One-Step No-ROX RT-qPCR Mix (AM06)

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AMPLIFYME SG One-Step No-ROX RT-qPCR Mix (AM06)

Wyczyść

Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on intercalating dsDNA binding dye chemistry: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers. AM06, AM06-100, AM06-500

Opis

The AMPLIFYME SG One-Step No-ROX RT-qPCR Mix is a convenient reaction mixture created for reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube. The use of high-affinity antibody for hot-start polymerase ensures higher specificity,by reducing formation of primer-dimer structures. It allows to obtain wider dynamic range by removing competition for reaction reagents, it also leads to higher sensitivity and reproducibility. Additionally, Mu-MLV Reverse Transcriptase and RNase Inhibitor are included in separate tubes. Precisely optimized buffer components ensures optimal conditions for reverse transcriptase and hot-start polymerase activity. Additionally RNase Inhibitor protects RNA from unspecific RNases. The AMPLIFYME SG One-Step No-ROX RT-qPCR Mix provides fast, highly specific one-step Real-Time RT-PCR results, giving consistent results across all commonly-used Real-Time PCR platforms. Features & advantages Versatile – excellent for various PCR conditions using different Real-Time PCR instruments Sensitive – reliable detection of low copies RNA targets Reproducible – consistent amplification across a wide dynamic range Specific – precisely selected anti-Taq antibody eliminates non-specific amplification Fast – accurate detection of molecular targets in as fast as 40 minutes (with either two- or three-step cycling profiles) Universal – reliable detection of RNA targets from broad range of samples

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